Carcinogen adsorbent prepared from DNA complex by -ray irradiation

Furusawa, Kazuya*; Kita, Erika*; Saeki, Toshihiko*; Nagasawa, Naotsugu; Nishi, Norio*; Dobashi, Toshiaki*

Journal of Biomaterials Science; Polymer Edition, 19(9), p.1159 - 1170, 2008/09

To insolubilize DNA for preparing a novel carcinogen adsorbent, effects of -ray irradiation on aqueous solutions of mixtures of DNA with cross-linking biopolymers (CBP): gelatin, bovine serum albumin, carboxymethyl cellulose and hydroxyethyl-cellulose, O-[2-hydroxy-3-(trimethyl ammonio)-propyl], chloride have been studied. DNA-CBP hybrid gels were successfully prepared from aqueous mixtures of DNA with CBP in a limited range of irradiation dose. Gel fraction (Gf) and swelling ratio (S) of the hybrid gels were measured. The Gf and S of hybrid gels increased and decreased, respectively, with increasing irradiated dose. Adsorption of one of carcinogen, acridine orange, was also examined for the gels. From the experimental results, the optimum condition for preparing insolubilized homogeneous DNA gels was determined.